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This chapter mainly centered on and defined the key and significant parameters in the liquid chromatography for the method development and optimization of an acceptable balance-indicating LC method and impurity profiling research. Each and each parameter which controls the purification of most of the natural compounds inclusive of drug, its precurs
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Analyte molecules partition between a liquid stationary stage and also the eluent. Just as in hydrophilic interaction chromatography (HILIC; a sub-technique in HPLC), this method separates analytes based upon dissimilarities in their polarity. HILIC most frequently utilizes a bonded polar stationary phase along with a cellular stage built principal